2.1.6-(d) sections of plant tissue showing the cell cycle and stages of mitosis PAG

 This can be considered a classroom PAG, but is also useful knowledge.

Preparation of stained squashes of cells from root tips

(Meristem tissue that can divide by mitosis for growth)

1) Sample preparation- wear gloves and forceps to handle the tips, which must be actively growing. Place into 5M hydrochloric acid, and rinse in cold water.

2) Cut the root tips- place onto microscopic slide

3) Staining- add 2-3 drops of stain and leave for two minutes. Use a mounted needle to spread the root tips into a thin layer.

4)Viewing the sample- Place the slide of the stage using the lowest power lens. On a lens power of x400, it should be possible to clearly see the chromosomes in the dividing cells.